RFMP Analysis, Gray, 1992

[Gregory M. Acland]

In 1992 Gray (Am J Hum Genet 1992 Feb;50(2):331-46) described detection
of DNA sequence polymorphisms as restriction-fragment melting
polymorphisms (RFMPs) in human genomic DNA using denaturing gradient gel
blots.  This seems to be a very attractive way of detecting gene specific
polymorphisms, particularly in species for which sequence is unlnown,
with resolution similar to pcr based technics. Has anyone had any
experience in using this, or variants thereof?
Alternatively, does anyone have an Email address for the author,  Dr MR
Gray at the Department of Obstetrics and Gynecology, Tufts University
School of Medicine, Boston, MA
Gregory M. Acland        	       	       	       	       	  (607)
256-5684; or  277-3044 [phone]
Center for Canine Genetics & Reproduction       	  (607) 256-5689; or
277-8399  [fax]
James A. Baker Institute for Animal Health      	   gma2 at cornell.edu
Cornell University, Ithaca, New York 14853