Two-step ligations (again).

[Dr R. Dalgleish]

I seem to recall that there was discussion recently on the best way
to ligate a fragment with one blunt end and one sticky end into a
vector. I think it involved performing a dilution part way through
and adding more ligase.

Can somebody summarise the info or point me in the right direction?

Thanks

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  Dr Raymond Dalgleish,          JANET: ray at uk.ac.le
  Department of Genetics,     INTERNET: ray at le.ac.uk
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