Sequencing with Degenerate primers?

SCOTTA at NCCCOT7.AGR.CA SCOTTA at NCCCOT7.AGR.CA
Thu May 12 12:09:37 EST 1994


Hi there,

I've been using a thermocycle sequencing kit for sequencing a PCR product
derived from a couple of potyvirus strains and was wondering if anyone had
any success using degenerate primers for sequencing? The primer I intend to
use has only a 4-fold degeneracy, and was wondering if it was suitable to use
for sequencing. If anyone could give me some advice, it would be really 
appreciated.

Thanks in advance,

Andrew Scott
=============================================================================
Agriculture Canada - Central Plant Health Laboratory
3851 Fallowfield Road
Nepean, Ontario
CANADA   K2H 8P9			Email: scotta at ncccot7.agr.ca
=============================================================================



More information about the Methods mailing list