?Semi-Dry Blot apparatus

Bruno Becker bbecker at iastate.edu
Thu May 12 10:21:52 EST 1994


In article <1994May9.144154.2214 at galileo.cc.rochester.edu>,
Tom Thatcher <ttha at troi.cc.rochester.edu> wrote:
>I am looking for recommendations on semi-dry blotters for
>Western blotting.  We used to have a Gelman Bio-Trans which
>used flat graphite plates and we loved it.  The plates are
>worn out and we need to replace it.  We are currently trying
>a BioRad unit which has been giving us some problems with
>uniformity and conditions.
>
>Which brand of semi-dry blotter do you like, and have had
>good success with?  Do you know of anyone that still sells
>graphite semi-dry blotters?
>
>Tom Thatcher
>University of Rochester
>ttha at troi.cc.rochester.edu
>
>
Try using the NovaBlot apparatus from LKB-Pharmacia. I've been using it with
good results for blotting of proteins over a wide range of mol. wt. (up to 300
kD). It does have graphite electrodes, which, however, seem to wear out after
100-200 blots (approx.).






-- 
Bruno Becker
Email: bbecker at iastate.edu
Phone (USA): (515)294-1985
Fax (USA): (515)294-4141



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