methods to homogenize E.coli

Zhiwei Wang zhiwei at
Sun May 15 19:28:59 EST 1994

Can somebody provide information on homogenizing E.coli?
I need to crush the cells to recover the recombinated
enzymes inside.  Beside sonication, are there any other 
good (I mean milder) methods?  On sonication, can you 
also comment on the conditions for preventing the
denaturation of the enzymes?
Please respond to this e-mail address.  I can seldom get to read 
the newsgroup.  Thank you.
Zhiwei Wang
Department of Zoology
University of Toronto
Toronto, Ontario
M5S 1A1

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