methods to homogenize E.coli
zhiwei at zoo.toronto.edu
Sun May 15 19:28:59 EST 1994
Can somebody provide information on homogenizing E.coli?
I need to crush the cells to recover the recombinated
enzymes inside. Beside sonication, are there any other
good (I mean milder) methods? On sonication, can you
also comment on the conditions for preventing the
denaturation of the enzymes?
Please respond to this e-mail address. I can seldom get to read
the newsgroup. Thank you.
Department of Zoology
University of Toronto
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