Degenerate primers

Jesus del Valle del_valle at MPIMG-Berlin-Dahlem.MPG.DE
Mon May 16 12:33:56 EST 1994


Hi. A friend of mine had the same problem a few weks ago. I had been reading 
Barnes' PNAS article so I suggested him to use a proof-reading polymerase 
instead of Taq Pol lacking the 3'-5' proof-reading ability in the hope of 
correcting possible mistakes at the 3'-end of the degenerate primers. It 
worked beautifully. He used Perkin Elmer's UlTma Pol.
Jesus 



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