Blunt ligation

David Hoffman dhoffman at spot.Colorado.EDU
Tue May 17 17:28:49 EST 1994


uxa.cso.uiuc.edu ( Dr. Scott Holt) writes:


>What condition and concentration is the best for a bunt end ligation?

I find that using a vector:insert ratio of 1:3 up to 1:8 (wt./wt.) in a volume
of 100 ul works well if you add 1/10 volume of 50% PEG-4000.

Dave Hoffman




More information about the Methods mailing list