uxa.cso.uiuc.edu ( Dr. Scott Holt) writes: >What condition and concentration is the best for a bunt end ligation? I find that using a vector:insert ratio of 1:3 up to 1:8 (wt./wt.) in a volume of 100 ul works well if you add 1/10 volume of 50% PEG-4000. Dave Hoffman