Subcloning from polyacrylamide gels
dhoffman at spot.Colorado.EDU
Tue May 17 17:30:52 EST 1994
jenfedaque at samba.cnb.uam.es writes:
>Does anyone know a simple and reliable method of subcloning DNA fragments
>form acrylamide mini-gels?
>I used to do it in agarose gels, but for small fragments, PAGE's resolution is
>better, so...I wonder if there is a convenient way to recover the DNA.
>(Please, without time-spending electroelutions...etc.)
I've had good luck (and results) subcloning from bacteriophage lambda clones
by working directly from the restriction enzyme digestions, without an inter-
mediate fragment prep of any sort. E-mail me if you have some questions.
> Please, reply to:
>Josefina Enfedaque josefina at farmacia.ub.es
>Univ. Barcelona (Spain)
More information about the Methods