ss DNA purification
Sarven Sabunciyan
sarven at org.ecc.ubc.ca
Wed May 18 17:22:16 EST 1994
Dear Netters!
Does anyone out there have a method for isolationg ss M13 phage DNA on a
cesium chloride gradient instead of a potassium iodide gradient? What I
am doing is in vivo excision from a Lambda ZapII library but the ss DNA I
isolate has a huge contamination of ds DNA (concluded from S1 nuclease
digestion). Any help is appreciated. Thanks in advance.
Sarven
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