Hints for PCR of A/T-rich sequences?

robin carper RCARPER%JHUHYG.BITNET at JHUVM.HCF.JHU.EDU
Wed May 18 07:15:48 EST 1994


I have to do a bunch of PCR on highly A/T rich sequences and was wondering
if anyone has done any optimization experiments for any of the zillion PCR
variables, as they relate to A/T sequences.  Lower extension temp? Higher salt?
Increase gelatin?  Any thoughts on this would be appreciated.

                        rcarper at jhuhyg.sph.jhu.edu

                                    Thanks much

                                        robin



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