LipofectAmine and Autofluorescence

vaughan at vaughan at
Wed May 18 11:27:37 EST 1994

Has anyone seen auto-fluorescent structures in their cells after 
transfection using GIBCO-BRL's LipofectAmine? It seems to be transfection
dependant, and the GIBCO people say that it's specific to LipofectAmine.
They admit to receiving calls about it, but have not yet figured out the
problem. They suspect that the reagent is complexing with something in the
media (phenol red?).
	Any thought welcome.
	Kevin Vaughan

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