BamHI Without Star Activity
smrodems at students.wisc.edu
Wed May 18 23:11:30 EST 1994
In article <1994May19.005402.28015 at vaxkiller.agi.org>,
vaxkiller.agi.org!rlynch (rick lynch) wrote:
> I am having some trouble getting a clean BamHI digest of a plasmid without any
> extra bands. I am using 5X excess of enzyme by units, and am using a standard
> buffer with 150 mM NaCl. It is important that I get only linear plasmid, and
> I can't gel purify the fragment for other reasons I won't get into now.
My first suggestion would be to use BamHI from another company than the one
your using. I have never had problems with BamHI from BRL. This enzyme
cuts in REact 3 which is 100mM NaCl. Maybe you buffer is too high in salt.
I have never used BamHI from NEB but several other enzymes from NEB work
well in my hands so they may be a good source as well.
Steve "Some day I will get the hell out of Wisconsin" Rodems
"Then I am here for the Lee family renioun ... shur-wajo-shur"
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