E. coli and SDS-PAGE

Virginia Dress Dress at biosci.arizona.edu
Thu May 19 17:39:02 EST 1994


In article <2rgiee$811 at dartvax.dartmouth.edu>, bob.gross at dartmouth.edu (Bob
Gross) wrote:
> 
> Your viscosity is from DNA. If you are just interested in proteins, you
> might sonicate the cells to open them, digest the DNA with DNAase, and
> then add the SDS etc. to prepare your sample.
> 
> Bob Gross
> Dept. of Biology, Dartmouth College
> bob.gross at dartmouth.edu

Also try diluting the cell pellet with more sample buffer, you don't say
how
dense the cell cultures are, but my guess is that you have PLENTY of
protein
there.

Virginia Dress



More information about the Methods mailing list