A spotty Northern

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Thu May 19 05:59:50 EST 1994

ar229 at FreeNet.Carleton.CA (Allison Haggarty) writes:

>...and 2) the spots are EVERYWHERE on the film,
>not just on top of the membrane. ....

Did you happen to put your exposure cassette next to another which had
someone's library/colony screens in it. Radiation comes out the back of
cassettes so if the two cassettes are back to back and one of them contains
something very hot and spotty it will also expose the film in your Northern
cassette. This would explain the appearance of spots everywhere on your film.

Frances Hannan
BILMS, Zoology, Cambridge, UK
flh at mole.bio.cam.ac.uk

More information about the Methods mailing list