Humidity

Martin Leach leach at mbcrr.harvard.edu
Mon May 23 05:58:45 EST 1994


In article <JEB.Harrison-230594180726 at 130.216.55.61>,
JEB.Harrison at auckland.ac.nz (Jane Harrison) wrote:

> I have a problem with my 96 well plates, the edges keep drying out even
> when I have put water in the provided holes to maintain some humidity.  I
> am growing virus which takes 5-7 days to infect fully under the growing
> conditions, and I can't seem to stop the plates starting to dry out in this
> time.  The cells require air flow so I can't parafilm the plates to keep
> the mositure in. 
> Any ideas welcome.
> Jane 
> 


Are u putting water in the incubator - either fill the base of the
incubator (some are made for this) or place it in a large tray

we had similar problems with 24well plates in incubator

Martin
-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
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