ss DNA purification

Andrew Taylor ataylor at fred.fhcrc.org
Mon May 23 16:10:55 EST 1994



>Does anyone out there have a method for isolationg ss M13 phage DNA on a
>cesium chloride gradient instead of a potassium iodide gradient?  What I
>am doing is in vivo excision from a Lambda ZapII library but the ss DNA I
>isolate has a huge contamination of ds DNA (concluded from S1 nuclease
>digestion).  Any help is appreciated.  Thanks in advance.

Is the M13 packaged in this system?  Purifying M13 phage (on a CsCl gradient) 
is a very good way to get it away from just about everything (except perhaps 
ribosomes) because the DNA-protein complex has a density very different from 
proteins or DNA.

Andrew




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