Mon May 23 18:10:29 EST 1994
I have been using Amersham in vitro site-directed mutagenesis
kit for a long time by using cloned M13-ssDNA as a template.
Suddenly, I had problems when made the transfection of
the final mutagenesis reaction. I used to XL1Blue MRF'
competent cells to do the transfection. I could not get
not plaque on plate. After the first time, I could not even
get plaque from the M13 ssDNA control DNA. I used the standard
protocol for making competent cells, and transfection.
Has anybody experieced similar situation. Or could anybody
tell me what is going on and give me some advice.
Thanks a lot.
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