One-Step Transformations

Michael G. Tencza tencza at med.unc.edu
Tue May 24 13:16:22 EST 1994


Hi Netters!

I have been trying to use the one-step transformation method (Chung etal.,
1989) to isolate constructs of 7.5-10.5kB (pBR322, HB101).  Although my
cells have taken vector without insert consistently well, I have had very
low efficiencies obtaining vectors with inserts as described.  These
constructs have since been isolated using electroporation.  Is there a
size limitation with this method in anyone's experience or is this just a
phenomenon based on Voodoo?

Thanks in advance, Mike.



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