air cyclers and fluorescent dyes

kalmar at sfu.ca kalmar at sfu.ca
Thu May 26 17:30:10 EST 1994


We recently utilized an Idaho air cycler for RAPD's with fluorescent
labelled oligos. At this time we investigated the effects of the light
source on the properties of the fluorescent dye (ABI Fam). We observed no
adverse effects due to photo bleaching. If I remember correctly, there was
some breakdown of the dye and it was as a result of heat. ABI confirmed for
us that this is normal.


Gabe Kalmar
Inst. Molecular Biology & Biochem
Simon Fraser Univ.





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