Blunt ligation

Manuel Simon ma at ABC.univie.ac.at
Fri May 27 18:53:56 EST 1994


In article <dhoffman.769213729 at spot.Colorado.EDU> dhoffman at spot.Colorado.EDU (David Hoffman) writes:
>From: dhoffman at spot.Colorado.EDU (David Hoffman)
>Subject: Re: Blunt ligation
>Date: Tue, 17 May 1994 22:28:49 GMT

>uxa.cso.uiuc.edu ( Dr. Scott Holt) writes:


>>What condition and concentration is the best for a bunt end ligation?

>I find that using a vector:insert ratio of 1:3 up to 1:8 (wt./wt.) in a volume
>of 100 ul works well if you add 1/10 volume of 50% PEG-4000.

>Dave Hoffman

High Dave,

Of course, by addition of PEG you can increase the probapility of 
inter-molecular reactions. You also could take small simply volumes. In my 
hands ligases apparentyl dont like PEG!.Manuel





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