DNA aggregation and Different. cDNA Display

dkim at unm.edu dkim at unm.edu
Fri May 27 10:24:09 EST 1994

Question posted about PCR amplified DNA in wells of gel for Diffrerential 
display PCR.

If you are using denaturing gels, you probably boil your PCR product prior to
loading. Could this be  causing coagulation of the different proteins in the
reaction mix?

Some people use native PAGE for DDPCR. Maybe you can try that out.

Daniel Kim  kim at flovax.lanl.gov

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