Formamide-less southern protocol for biotinylated probes???

Parke K. Flick ch994 at cleveland.Freenet.Edu
Fri May 27 12:33:21 EST 1994

Try the USB Gene Images Hybridization Buffer (Church and Gilbert)
buffer, which does not have formamide.  Results with biotinylated
probes, both oligo and longer, are excellent, especially with
Northern blots.
P. Flick
Parke Flick, Ph.D.
U.S. Biochemical Corp.
Cleveland, OH  44128

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