How necessary to prevent self-ligation using CIP?

[Huanquan Zheng]

Yes, to prevent recircularization of lineared vector, it is abselutely
needed.

But when you guys do the cloning, in the following cases, is it
absolutely necessary to use CIP to prevent self-ligation of vertor?

1. vector digested with BamH I & Kpn I with which insert will be digested too.
2. blunt end vector (which blunt end insert will be ligated into)


--
	Huanquan Zheng 	~{V#;7H*#,KD4(HK#,;lHUWS5D#,~} 
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