How necessary to prevent self-ligation using CIP?

Huanquan Zheng zheng at cc.Helsinki.FI
Tue May 31 07:29:55 EST 1994


Yes, to prevent recircularization of lineared vector, it is abselutely
needed.

But when you guys do the cloning, in the following cases, is it
absolutely necessary to use CIP to prevent self-ligation of vertor?

1. vector digested with BamH I & Kpn I with which insert will be digested too.
2. blunt end vector (which blunt end insert will be ligated into)


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	Huanquan Zheng 	郑环泉,四川人,混日子的, 
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