pUC18 as expression vector
Martin at gene.unp.ac.za
Tue May 31 04:03:27 EST 1994
I am interested to know what success people have had at getting expression
in E. coli of bacterial genes cloned into pUC vectors with the genes under
control of the lac promotor. I am particularly interested in the expression
of genes from Pseudomonas sp. - I would also be interested in knowing
whether anybody has any experience with the expression in E. coli of genes (
cloned into any vector) from gram negative aerobic bacteria (suc as the
Pseudomonads) under the control of the genes' native promotors.
I would be extremely greatful for any useful information.
Department of Genetics
University of Natal, Pietermaritzberg
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