Can I successfully blunt it?

Huanquan Zheng zheng at cc.Helsinki.FI
Tue May 31 08:03:24 EST 1994


I have an insert which prosess both 5' and 3' overhang protruding in
upstream, to blunt this insert, the following has been planed to do:

20 ul reagent: 
Klenow fragment enzyme 3U
Insert		       3g
dNTPs		      2mM final conc (high?)
10x klenow buffer     2ul

30 min at RT

Can I successfully fill in recessed 3' terminus in downstream and
remove that 3' protruding in upstream after above treatment?

Some protocol said I can, some said I can't. so I am begging the
advises of experienced hand here.
 
I do not prefer to use S1 or mung-bean.

--
	Huanquan Zheng 	郑环泉,四川人,混日子的, 
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