Message Selection in B Subtilus

DAVID MITCHELL mitcheld at rocbi.DNET.roche.com
Tue May 31 10:02:23 EST 1994


Dear Netters,

A colleague of mine is having background trouble with ribosomal RNA when
probing Notherns of B subtilus.  She would like to select for mRNA but has been
told that it is not possible with poly-A selection as bacterial message has no
(or only small) poly-T tracts.  Is this correct? and does anybody have any
experience in selecting for mRNA in B subtilis.  

You can post answers directly to her (Cassandra) at
vocklerc at rocbi.dnet.roche.com or me (mitcheld at rocbi.dnet.roche.com)

Many thanks David




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