Quantitative protein determinations in plant extracts

Ragnar Flengsrud biorf at nlh10.nlh.no
Tue Nov 1 02:13:39 EST 1994


In article <CyEDoz.Du8 at phibred.com>, bipin dalmia <dalmiabk at phibred.com>
wrote:

> 
> what is an absolute method of protein determination in plant extracts
> besides gravimetric analysis? in particular, does anyone have data on how
> nitrogen combustion, Kjeldahl or any other method compare when
> determining total protein content in any kind of crude preparations? we
> are getting pretty large differences between lowry (folin), BCA and
> bradford assays. these were done with BSA as a standard, and all buffer
> inteferences were eliminated.
> 
> bip

Bip,

Amino acid analysis is the only reliable method I know of here. Try doing a
BCA-test on the same sample(s). This will give you a correlation factor, so
you dont have to do amino acid analysis repeatedly.

Regards,

-- 
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RAGNAR FLENGSRUD                                Ph.: + 47-64947710
Institute of Biotechnological Sciences          Fax :+ 47-64947720
P.O. 5040, Agricultural University of Norway   
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