Odd problem with RAPD sequencing

Michael Cooley szcooley at dale.ucdavis.edu
Tue Nov 1 16:42:16 EST 1994


Robert Rumpf (Rumpf.1 at osu.edu) wrote:
: I'm posting this for a friend...he's successfully amplified a RAPD band
: and cloned it, then sequenced it using vector primers.  He went ahead and
: designed primers for the RAPD based on the sequences he obtained and now
: can't amplify using these specific primers!  Anyone have a problem similar
: to this or perhaps have an explanation for it?

Its not an unusual problem to have difficulty going to a SCAR. Did he get 
no bands amplified at all or just the specific band he sequenced?


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