Homemade GeneClean kits ???

[Michael Cooley]

Marc Goldstein (ez001427 at dale.ucdavis.edu) wrote:
: fnord (jpa6r at faraday.clas.Virginia.EDU) wrote:

: : 	I was told that some labs make their own reagents for 
: : silicon based DNA purification (i.e., GeneClean kits) including the
: : glass beads. Does anyone know of any protocols?  I would appreciate
: : any pointers to the original literature describing the method.

: : 		Thanks,

: : 			jpa6r at virginia.edu

: : --
: I've never made the glass beads before, but I have had opportunity to 
: make up a batch of the sodium iodide.  First time I tried it, my yield 
: was terrible.  I was then recommended to filter it through a very thick 
: layer of glass fiber filters.  After doing this, the solution worked just 
: fine.  I presume there is a contaminant in commercially available sodium 
: iodide that interferes with the DNA-glass binding.  Filtering through 
: GFFs titrates out this contaminant.

: Marc Goldstein
: Section of plant Biology
: University of California Davis
: magoldstein at ucdavis.edu


The protocols are in FAQ. My reagents performed at twice the yield of 
GeneClean.


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