Advise needed on DNA strand-separation

Baohua Huang bhuang at magnus.acs.ohio-state.edu
Tue Nov 1 12:11:42 EST 1994


Hi, folks. I would like to get some advise on DNA strand separation. I have 1 
mg of dsDNA of 150 nt long obtained by PCR and would like to separate each 
strand into ssDNA. Due to the large scale, the avidin-agrose method didn't work
well (here one strand of the dsDNA contains a bitin at terminus). I intend to
separate the dsDNA by denaturing PAGE (sequencing gel)because upon
denaturation, one strand has the extra biotin part which would slow down its
migration.
Any suggstions?

Thanks

Baohua
Ohio State University



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