Advise needed on DNA strand-separation

Baohua Huang bhuang at
Tue Nov 1 12:11:42 EST 1994

Hi, folks. I would like to get some advise on DNA strand separation. I have 1 
mg of dsDNA of 150 nt long obtained by PCR and would like to separate each 
strand into ssDNA. Due to the large scale, the avidin-agrose method didn't work
well (here one strand of the dsDNA contains a bitin at terminus). I intend to
separate the dsDNA by denaturing PAGE (sequencing gel)because upon
denaturation, one strand has the extra biotin part which would slow down its
Any suggstions?


Ohio State University

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