Odd problem with RAPD sequencing

Robert Rumpf Rumpf.1 at osu.edu
Tue Nov 1 11:14:03 EST 1994


I'm posting this for a friend...he's successfully amplified a RAPD band
and cloned it, then sequenced it using vector primers.  He went ahead and
designed primers for the RAPD based on the sequences he obtained and now
can't amplify using these specific primers!  Anyone have a problem similar
to this or perhaps have an explanation for it?



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