Clontech Two-hybrid libraries.

[Simon E Plyte]

We recently bought the Matchmaker two-hybrid system from Clontech together with
a suitable fusion library. Two labs here have tried using this system
without any luck. 

When we screen the library we get between 100-400 colnies that grow
on His-, trp-, leu- plates. However, none of these turn blue in the 
X-gal assay. Clontech assure us that at least half shoud turn blue.

Question: Has anyone out there had any luck cloning an interacting 
protein using the matchmaker system together with a Clontech library.

Cheers
Simon