Use of Amersham's Hybond N+

Emma Macfarlane emma at HERA.MED.UTORONTO.CA
Wed Nov 2 12:03:12 EST 1994

I have also used Hybond N+ on a regular basis for genomic and plasmid DNA 
(Southerns, colony blots, dot blots) and it has worked just fine.

On 1 Nov 1994, Michael Cooley wrote:

> Grace Mendoza (mendoz at wrote:
> : Our laboratory just recently heard that DNA does not transfer real well to
> : Amersham's Hybond N+ filters.  Has anybody else heard this?  It is
> : supposedly better to use for transfer of RNA not DNA. Does anybody have
> : comments, experiences with using Hybond?  Thanks.
> : Grace Mendoza
> : mendoz at
> : General Atomics
> We use it regulary here for genomic DNA and have not experienced any 
> difficulties.

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