HA epitope tag problems

PJOB obrien at pharm.med.upenn.edu
Wed Nov 2 14:51:13 EST 1994


In article <spang-3110941614350001 at genmac13.biochem.mpg.de>,
spang at vms.biochem.mpg.de (Anne Spang) wrote:

> Hi Peter!
> Are you sure that your tagged protein is made? Is it possible, that your
> protein is degraded?
> 
> Anne

Yes, we have confirmed the function of the receptor by Inositol Phosphate
assay and Ca++ transients and it's expression on the surface by FACS.  We
just can't IP or western blot it.  The DNA sequence checks out o.k. too.



More information about the Methods mailing list