HA epitope tag problems
obrien at pharm.med.upenn.edu
Wed Nov 2 14:51:13 EST 1994
In article <spang-3110941614350001 at genmac13.biochem.mpg.de>,
spang at vms.biochem.mpg.de (Anne Spang) wrote:
> Hi Peter!
> Are you sure that your tagged protein is made? Is it possible, that your
> protein is degraded?
Yes, we have confirmed the function of the receptor by Inositol Phosphate
assay and Ca++ transients and it's expression on the surface by FACS. We
just can't IP or western blot it. The DNA sequence checks out o.k. too.
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