Cloning with LambdaGEM-12
devitta at uk.ac.birmingham
Wed Nov 2 06:24:37 EST 1994
LambdaGEM-12 Xho I half-site arms cloning system.
In this system LambdaGEM-12 has been digested with Xho I, partially
filled-in with dTTP and dCTP and dephosphorylated. This allows it to
ligate specifically with Mbo I or Sau 3A I digested genomic DNA which has
been partially filled-in with dATP and dGTP.
Using this cloning system (from Promega) I have been obtaining
unusually high levels of background when vector DNA is ligated and
packaged in the absence of insert genomic DNA. Typically 20,000
pfu/microgram of vector DNA.
Has anybody else had similar problems?? I would appreciate any advice
More information about the Methods