Direct sequencing of lambda phage dna

rmewing at vax.oxford.ac.uk rmewing at vax.oxford.ac.uk
Wed Nov 2 09:49:26 EST 1994


Anyone have experience with direct sequencing of lambda phage DNA?
How easy is it...which isotope should I use, and do I need to end-label my
primer, which enzyme etc.

Is it difficult to get clear results and if so would I be better either
subcloning into plasmids or PCR-amplifying and direct sequencing of the product?


Thanks,
Rob Ewing.



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