Loading Long Ranger gels...

Mic Chaudoir mic at nwu.edu
Wed Nov 2 16:07:45 EST 1994


In article <395651$jag at eldborg.rhi.hi.is>, php at rhi.hi.is (Petur Henry
Petersen) wrote:

>         I was wondering... When running Long Ranger Sequencing gel (6%)
> I usually wait until the gel is around 45 degr and run it at about 45-50 degr
> (Celcius of course :) ). In the Long Ranger leaflet they say one has only
> to prerun the gels for 10 min. After 10 min prerun my gels are about 35 degr.
> Should I load at 35 or wait as I usually do? I am trying to get sharper
> bands and one of the factors I am concerned about is the heat of the gels..
> 
>         Could anyone tell me what they do? I should mention that I am running
> sequenced PCR products and they show litle secondary structure and that I
> use biotinlabeled primers to get ssDNA for sequencing...
> 
>         Petur Henry Petersen DeptmBiology U.Iceland

I always just run the gel 20 minutes before loading.

-- 
mic
mic at nwu.edu

"DOS is proof that PC users can take a joke"



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