a problem in protein purification

Ding Ming ming at ahabs.wisc.edu
Thu Nov 3 13:40:54 EST 1994


In article <9411031154.AA36057 at bono.oci.utoronto.ca>,
jwoodget at oci.utoronto.ca (Jim Woodgett) wrote:


> it?).  There are three possibilities you could try.  


Jim,

there is at least one more possibility he/she could try:)
Dr. Richard Burgess's group had developed a "soft elution" procedure when
they were working on RNA polymerase a few years ago. The basic discovery
was that IgG bound protein can be eluted from the affinity column by a
solution of ammonia sulfate. This procedure was used to solve one of the
problems I encountered at a similar situation. I am sorry that I cannot
give you the reference at this movement, but you can find it by doing a
little bit search.

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Ding Ming
University of Wisconsin-Madison
Telephone: (608)265-3544     Fax: (608)262-7420
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