cell surface biotinylation

uvsbgsm at Msu.oscs.montana.edu uvsbgsm at Msu.oscs.montana.edu
Thu Nov 3 14:38:58 EST 1994


I have been trying to cell surface biotinylate membrane proteins with very
little efficiency.  Radiolabeled monoclonals bound to surface proteins give a
higher signal, so I really think I have an efficiency problem.  The protein is
a highly glycosylated type I protein expressed in CHO cells.  Any
methods/advice would be welcome.  

Thanks!



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