help for oligo hybridization

Janet E. Rubin jrubi02 at unix.cc.emory.edu
Thu Nov 3 10:14:40 EST 1994


Perhaps I missed the beginning of this thread, but do you wish to 
hybridize Northerns or Southerns with your oligos?  We've had success 
with Northerns when the oligos are longer - 48mer or more.  But with 
Southerns, even 20mers work great.  We've p32 and dig labelled for 
Southerns with these short oligos.  Is this what you're after?




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