Loading Long Ranger gels...

Petur Henry Petersen php at rhi.hi.is
Thu Nov 3 08:07:06 EST 1994


In <mic-0211941507450001 at mac125.physio.nwu.edu> mic at nwu.edu (Mic Chaudoir) writes:

>In article <395651$jag at eldborg.rhi.hi.is>, php at rhi.hi.is (Petur Henry
>Petersen) wrote:

>>         I was wondering... When running Long Ranger Sequencing gel (6%)
>> I usually wait until the gel is around 45 degr and run it at about 45-50 degr
>> (Celcius of course :) ). In the Long Ranger leaflet they say one has only
>> to prerun the gels for 10 min. After 10 min prerun my gels are about 35 degr.
>> Should I load at 35 or wait as I usually do? I am trying to get sharper
>> bands and one of the factors I am concerned about is the heat of the gels..
>> 
>>         Could anyone tell me what they do? I should mention that I am running
>> sequenced PCR products and they show litle secondary structure and that I
>> use biotinlabeled primers to get ssDNA for sequencing...
>> 
>>         Petur Henry Petersen DeptmBiology U.Iceland

>I always just run the gel 20 minutes before loading.


	Yeasterday (wrong spelling?) I did run 2 gels. One of those i
preran for 20 min and loaded it at 35 degr. The other one i ran longer
and loaded at 43 degr.

	When I develop those I will post the results (if any). In the
meantime I will continue loaidng them at 44-45 degr.

	happy days! php U.Iceland



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