UV Source for SYBR GREEN?
J.Carnwath
carnwath at tzv.fal.de
Fri Nov 4 06:10:07 EST 1994
Greetings:
Could someone who is using SYBR GREEN 1 for Gel photography tell me what
they are using as a 254 UV light source?
The technical information from Molecular Probes Inc. states that 254 nm
epi-illumination is better than 300 nm transillumination. We need to
purchase a UV source to properly evaluate the SYBR GREEN and could
purchase an epi-source if this is better. I imagine that if the UV
filter on the camera is not 100% effective, epi-illumination would be
better, especially for the higher energy 254 source.
Any opinions or first hand experience?
Thanks,
Joe
--
Joseph Carnwath carnwath at tzv.fal.de
Inst. fuer Tierzucht und Tierverhalten
31535 Neustadt Tel: (5043) 871-134
Germany Fax: (5034) 871-143
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