Genomic DNA from E.coli without phenol or chloroform??

Klaus Salger salger at
Fri Nov 4 20:58:44 EST 1994

Gabriel Dorado (bb1dopeg at wrote:
: Anybody knows a reliable method to obtain genomic DNA from E coli
: without the need of hazardous substances like phenol or chloroform? The
: DNA should be suitable for PCR amplification. Any kit available? I have
: read the new Puregene Isolation Kits from Flowgene can do it. Anybody
: has tried them?

There was a thread about PCRing whole E.coli cells picked directly from
the plate without purification. People did this to screen for plasmids
w/o inserts. To do the same with genomic sequences would be much more
difficult of course but maybe somebody tried it. If not - maybe you want
to try it.

Klaus Salger                phone : ++49 (0)89 5902 -502
Zoologisches Institut       FAX   :                 -450
AG MacWilliams              e-mail: salger at
Luisenstr. 14
80333 Muenchen

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