tagging membrane proteins
ndavid at bcm.tmc.edu
Fri Nov 4 13:32:51 EST 1994
I'm trying to tag a membrane protein so I could do immunocytochemistry. I
tried by adding an epitope sequence (Ab AU1 from BAbCo) in one of my PCR
subcloning the thing. It didn't seem to work. I've seen some people here
using HA. My questions are: a) How do you put the HA epitope in frame, (N
or C end)?
b) Are there polyclonal Abs available for that or
other tag epitope?
c) Other possible epitopes?
D) Something I don't know and I should?
Thanks a lot to everybody there asking and answering (I'm new in this net)
Baylor College of Medicine
E-Mail: rs690935 at mbcr.bcm.tmc.edu
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