DNA strand separation

rnwil003 at uctvax.uct.ac.za rnwil003 at uctvax.uct.ac.za
Fri Nov 4 10:57:35 EST 1994


RE STRAND SEPERATION. CHECK OUT BIOTECHNIQUES VOL, 17, PG 782. (1994)
METHOD USES MAGNETIC BEADS THAT HAVE STREPTAVIDIN BOUND TO THEM. THIS BINDS TO
BIOTIN ON ONE PRIMER. MAGNETISED BEADS EASY TO MANIPULATE. STRAND SEPERATION BY
0.1M NAOH. BOTH STRANDS EASILY PURIFIED. PRODUCT GOOD ENOUGH TO SEQUENCE WITH
SO MUST BE OK.
WILLIAM



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