immunostaining COS-7 cells

Jeffrey D. Bray jbdq+ at andrew.cmu.edu
Fri Nov 4 10:08:30 EST 1994


Hi,

I wish to transfect COS-7 cells with 2 different expression vector
plasmids containing the tropomyosin and NM/SM myosin light chain tagged
with the HA-epitope. I would then like to perform immunofluorescence to
see if there is correct localization of these gene products.  Does
anyone have a good protocol for immunostaining COS-7's or stress fiber
proteins.  Thanks in advance.

Jeff Bray CMU grad student <jbdq+ at andrew.cmu.edu> 



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