Smallest Cloning Vector and Selectable Marker ...
vam2 at midway.uchicago.edu
Fri Nov 4 10:05:07 EST 1994
In article <agpfxm-0411940816520001 at gra79.grasslands.cri.nz>,
agpfxm at pnv.palm.cri.nz (Ferenc Marincs) wrote:
> Hi, Viraj
> Do you know any source for the plasmid pMOB. Please let me know, I would
> need it.
> Thanks, Frank
> Ferenc Marincs
> AgResearch, Private Bag 11008, Palmerston North, New Zealand
> Phone: +64-6-356 8019, Fax: +64-6-351 8032
> E-mail: agpfxm at pnv.palm.cri.nz
Hi Frank (and others),
I should have provided the reference describing this vector more fully so
you can determine whether you need it. The ref. describing the
construction of this vector is:
Strathman, et al. Transposon-facilitated DNA sequencing.
Proceedings of the National Academny of Sciences U.S.A. (1991) 88:1247
from my previous post:
> > pMOB is a 1.8 kb stripped-down BluescriptII. It contains the full MCS,
> > T3/T7 RNA pol. binding sites, B-lactamase gene for ampicillin resistance
> > and an origin of replication. I hope this helps.
Dept. of Organismal Biology and Anatomy
University of Chicago
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