UV Source for SYBR GREEN?

dhanzel at delphi.com dhanzel at delphi.com
Sat Nov 5 01:06:38 EST 1994


J.Carnwath <carnwath at tzv.fal.de> writes:
 
>Could someone who is using SYBR GREEN 1 for Gel photography tell me what
>they are using as a 254 UV light source?  
>
>The technical information from Molecular Probes Inc. states that 254 nm
>epi-illumination is better than 300 nm transillumination.  We need to
>purchase a UV source to properly evaluate the SYBR GREEN and could
>purchase an epi-source if this is better.  I imagine that if the UV
>filter on the camera is not 100% effective, epi-illumination would be
>better, especially for the higher energy 254 source.
 
	For what it is worth I believe that SYBR Green is more efficiently
excited around 490nm. [e.g. 488 line of an Ar laser]
david hanzel
hanzel at mdyn.com
(Molecular Dynamics by day, Delphi by night) opinions are mine alone!



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