oligo purification on hplc

Robert Nelson ADEVICE at ix.netcom.com
Sat Nov 5 19:10:09 EST 1994


In <Picard.53.783987681 at nih.gov> Picard at nih.gov (John Cooper) writes: 

>
>Anyone use HPLCs to purify oligos? What kind of columm do you use?
>Gradient?. New to this technique (HPLC) so any advice appreciated...
>
>John Cooper
>Picard at helix.nih.gov
>John Cooper, MD
>National Inst. of Health
>Picard at helix.nih.gov
>

Consider Capillary Electrophoresis to check the purity of any oligo 
prep.  Frequently, a single peak on reversed phase LC will result in 
multiple peaks on CE.

Regards,
Robert Nelson



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