How to Calculate Oligo MW?

Roger Wiegand rcwieg at ccmail.monsanto.com
Sat Nov 5 13:10:58 EST 1994


In article <1994Nov3.140526.1 at mcvax4.d48.lilly.com>,
books at mcvax4.d48.lilly.com wrote:

> We are looking for a formula to calculate the exact MW for oligonucleotides.
> The oligo will have a 5'-hydroxy group. We can estimate oligonucleotide MW
> if we use 330 as the average MW for each base but we need a exact number. 
> Thanks DPSMITH smith_dennis_p at lilly.com

Generally one adds up the atomic weights of each of the atoms in a
molecule to obtain a molecular weight, c=12, o=16, h=1, etc. You probably
don't need to worry about the small differences due to minor isotopes.

-- 
Thanks,
Roger Wiegand

rcwieg at ccmail.monsanto.com



More information about the Methods mailing list